IL-2 anticorps (Biotin)

N° du produit ABIN115871

L’anticorps Lapin Polyclonal anti-IL-2 a été validé pour WB et EIA. Il convient pour détecter IL-2 dans des échantillons de Souris.

Aperçu rapide pour IL-2 anticorps (Biotin) (ABIN115871)

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-2 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-IL-2 anticorps (Biotin)

Specificité: Detects Murine IL-2.

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant mIL-2.

Information d'application

Indications d'application: ELISA: (Direct): To detect mIL-2 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIL-2. (Sandwich): To detect mIL-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine IL-2 (PP009P) as a capture antibody,allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-2. Western Blot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C under subdued light for one month or (in aliquots) at -20 °C for longer. This product is phototsensitive and should be protected from light.

Détails sur IL-2

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: Interleukin-2 / IL2

Sujet: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF

ID gène: 16183

NCBI Accession: NP_032392

UniProt: P04351

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation